Part:BBa_K5181017

pRGPDuo3_CPR_P450

This part is designed to express two enzymes, P450 and CPR, under the same two promoters so that the relative proportion of enzymes is the same.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4543
  Illegal XbaI site found at 152
  Illegal SpeI site found at 3137
  Illegal PstI site found at 140
  Illegal PstI site found at 461
  Illegal PstI site found at 1139
  Illegal PstI site found at 3811
  Illegal PstI site found at 4090
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4543
  Illegal NheI site found at 3391
  Illegal SpeI site found at 3137
  Illegal PstI site found at 140
  Illegal PstI site found at 461
  Illegal PstI site found at 1139
  Illegal PstI site found at 3811
  Illegal PstI site found at 4090
  Illegal NotI site found at 5494
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4543
  Illegal BglII site found at 4427
  Illegal BglII site found at 6065
  Illegal BamHI site found at 158
  Illegal XhoI site found at 1550
  Illegal XhoI site found at 1696
  Illegal XhoI site found at 5700
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4543
  Illegal XbaI site found at 152
  Illegal SpeI site found at 3137
  Illegal PstI site found at 140
  Illegal PstI site found at 461
  Illegal PstI site found at 1139
  Illegal PstI site found at 3811
  Illegal PstI site found at 4090
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4543
  Illegal XbaI site found at 152
  Illegal SpeI site found at 3137
  Illegal PstI site found at 140
  Illegal PstI site found at 461
  Illegal PstI site found at 1139
  Illegal PstI site found at 3811
  Illegal PstI site found at 4090
  Illegal NgoMIV site found at 590
  Illegal NgoMIV site found at 5429
  Illegal NgoMIV site found at 7101
  
• 1000
  COMPATIBLE WITH RFC[1000]